Quantitative PCR (q-PCR)

Transcript

MARION WOODCOCK What we can use real- time PCR for is to quantify how many copies of our transgene have been incorporated into the genome of our cell line. And real- time PCR is a great tool for that.

The real- time PCR machine is a standard PCR but with the incorporation of a dye, a fluorescent dye so that we can see the amplification of the DNA product live on screen as the reaction carries out.

So we can run a standard curve where we know how many copies are in our standard curve and compare that back to our transgene or our cell line and work out from there how many copies of the gene is incorporated.